Combined DIA/DDA
Proteomics
The gold standard for comprehensive, quantitative proteomics and phosphoproteomics. Get the breadth of DIA, the depth of DDA, and optional phosphosite mapping — all in one integrated workflow.
Service Overview
Get the best of both worlds — the breadth of DIA with the depth of DDA — plus optional phosphoproteomics to track post-translational modifications. This combined approach identifies and quantifies thousands of proteins while uncovering dynamic phosphorylation sites that control protein function.
Use it to map signaling pathways, discover disease biomarkers, or understand how cells respond to stress, drugs, or environmental changes. Our experienced team handles complex phosphopeptide enrichment and provides expert statistical analysis and pathway interpretation.
Best For
Pathway mapping and signaling analysis
Phosphoproteome discovery and site localization
Differential protein abundance studies
PTM-driven functional analysis
Comprehensive multi-omics studies
Researchers asking: what changed and why?
Deliverables
- → Protein identification and quantification tables
- → Phosphosite mapping and localization data
- → Differential abundance analysis with statistical metrics
- → Pathway and GO enrichment analysis
- → Heatmaps
- → PCA plots
- → and clustering analysis
- → Optional: Custom visualizations and raw MS data files
How It Works
Submit samples with our intake form
specifying whether phosphoproteomics is required.
Samples undergo SP3 preparation; phosphopeptide enrichment is performed where applicable.
Both DIA and DDA acquisition are run on the same samples to maximise protein coverage and identification confidence.
DIA data provides robust quantification; DDA provides deep sequence coverage and PTM localisation.
Our team performs differential abundance analysis and pathway enrichment using your experimental design.
You receive a comprehensive data package including all tables
statistical outputs
and optional custom figures.
Phosphoproteomics Add-On
Our phosphopeptide enrichment workflow maps serine, threonine, and tyrosine phosphorylation sites with high confidence and localisation scoring. Ideal for studying kinase networks, drug responses, and cell signalling dynamics.
Turnaround
Results typically delivered within 14–21 business days of sample receipt. Complex projects with phosphoproteomics may require additional time.
Platform
Combined DIA and DDA acquisition on high-resolution mass spectrometry. SP3 sample prep with optional phosphopeptide enrichment.
Analysis Included
Differential abundance analysis, pathway enrichment, GO term analysis, PCA, and heatmaps included as standard. Custom analyses available.
Pricing
$700 CAD per sample. Pricing varies based on sample number, phosphoproteomics inclusion, and analysis depth.
Sample Requirements
Cell lysates, tissue homogenates, or purified proteins. Minimum input quantities vary. Contact us before shipping.
Ready to submit samples?
Fill out our intake form and our team will follow up within 1 business day.
Submit a Service Request